One of the most critical environmental concerns is hydrocarbons of petroleum origin, which pose enormous challenges and risks for soil ecology, water resources, and human health, especially for those regions where fuel and oil handling activities are performed. The major aim of this study is to evaluate an integrated bioremediation technique using environmental optimization and natural enhancement materials for the degradation of petroleum hydrocarbons. A series of laboratory-scale batch experiments were carried out using hydrocarbon-degrading microorganisms isolated from hydrocarbon-contaminated soils. The degradation of hydrocarbon compounds was done under varying pH and temperature conditions, as well as varying combinations of amendment materials. The effectiveness of the degradation was monitored by observing the concentration of TPH, microbial density, and health parameters of the contaminated soil. The results revealed that the degradation of petroleum hydrocarbon compounds is possible in a temperature that is mild and in an almost neutral pH environment. The activated charcoal and bile salts work in combination to degrade the TPH compounds as efficiently as possible. The density of microbes was increased through the addition of compost and plant extracts, leading to the efficient degradation of the petroleum hydrocarbon compounds. It should be noted that the control of petroleum hydrocarbon compounds is possible through the use of the combination of natural substances and environmental control techniques.................... Keywords: ...............Bioremediation, Petroleum Hydrocarbons, Soil Contamination, Biosurf