This study aimed to isolate fungal strains from oil-contaminated soil in Libyan oil fields and from selected car workshops and oil-change centres, to evaluate their potential in the biodegradation of crude oil, and to determine their hydrocarbon-degradation capacity. Six fungal strains were identified and isolated: Aspergillus fumigatus, Aspergillus clavatus, Aspergillus terreus, Aspergillus oryzae, Penicillium sp1 and Penicillium sp2. The growth performance of these isolates was tested on Potato Dextrose Agar (PDA) medium supplemented with different concentrations of crude oil (1%, 3% and 5% v/v) A.clavatus exhibited the highest growth diameter, whilst A. terreus exhibited the lowest growth diameter at all concentrations. Biodegradation performance was also measured using the gravimetric method, and oil degradation rates at concentrations of 1%, 3% and 5% in the medium were: 90%, 78% and 60%, respectively, when using A. fumigatus. In contrast, Penicillium sp1, which recorded the lowest consumption rates; this is attributed to the inhibition of microbial enzyme activity at high contamination levels. The results demonstrate the efficacy of local Aspergillus isolates as promising candidates for the remediation of oil-contaminated areas............ This scientific manuscript was presented at the sessions of the International Renewable Energy, Gas, Oil and Climate Change Conference "iREGO" in the period of April 25-27, 2026. Tripoli - Libya............ Keywords:..................Crude Oil; Tibesti Field ; Aspergillus ; Penicillium ; Hydrocarbon Degradation.